cell lineage reconstruction
Recent technological advancements in light-sheet microscopy have opened up exciting new opportunities for in toto imaging of whole developmental arcs at the cellular level. With the help of advanced fluorescent proteins, light-sheet microscopy enables the reconstruction of the entire embryonic development at a cellular level, allowing for image-based lineage reconstruction.
These two datasets were acquired on our OpenSiMView light-sheet microscope. You can explore the full timelapse image data here:
These two datasets were acquired on our DaXi light-sheet microscope. You can explore the full timelapse image data here:
Note: We are planning to significantly expand this aspect of our ressource both in terms of data and accessibility. Stay tuned!
You can find the imaging and tracking data for Timelapse 1 here. Using our napari plugin for in silico fate mapping you can mark cells of interest and follow them over time. See video of demo below: